ha-tagged protein cat. Search Results


bfl1  (Sino Biological)
90
Sino Biological bfl1
Bfl1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/Human+Bcl2A1%2FBfl-1+transcript+variant+1+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-HA+tag/pmc05320539-156-8-21
Average 90 stars, based on 1 article reviews
bfl1 - by Bioz Stars, 2026-10
90/100 stars
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hatag  (Proteintech)
96
Proteintech hatag
Hatag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/HA+Tag+Antibody/pm38072043-183-31-32
Average 96 stars, based on 1 article reviews
hatag - by Bioz Stars, 2026-10
96/100 stars
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ha tagged protein purification kit  (MBL Life science)
90
MBL Life science ha tagged protein purification kit

Ha Tagged Protein Purification Kit, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/ha+tagged+protein+purification+kit/pmc08553338-37-15-7
Average 90 stars, based on 1 article reviews
ha tagged protein purification kit - by Bioz Stars, 2026-10
90/100 stars
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ha tagged ubiquitin  (R&D Systems)
95
R&D Systems ha tagged ubiquitin

Ha Tagged Ubiquitin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/Recombinant+Human+HA-Ubiquitin+Protein%2C+CF/pmc12148440-276-23-25
Average 95 stars, based on 1 article reviews
ha tagged ubiquitin - by Bioz Stars, 2026-10
95/100 stars
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ubiquitin  (Addgene inc)
96
Addgene inc ubiquitin
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Ubiquitin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/HA-Ubiquitin+(Plasmid+%2318712)/pmc07729981-157-1-17
Average 96 stars, based on 1 article reviews
ubiquitin - by Bioz Stars, 2026-10
96/100 stars
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lys k 63 ubiquitin  (Addgene inc)
94
Addgene inc lys k 63 ubiquitin
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Lys K 63 Ubiquitin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/pRK5-HA-Ubiquitin-K63+(Plasmid+%2317606)/pmc09373805-36-16-24
Average 94 stars, based on 1 article reviews
lys k 63 ubiquitin - by Bioz Stars, 2026-10
94/100 stars
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lys k 48 ubiquitin  (Addgene inc)
94
Addgene inc lys k 48 ubiquitin
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Lys K 48 Ubiquitin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/pRK5-HA-Ubiquitin-K48+(Plasmid+%2317605)/pmc09373805-36-9-24
Average 94 stars, based on 1 article reviews
lys k 48 ubiquitin - by Bioz Stars, 2026-10
94/100 stars
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prk5 ha ubiquitin wt  (Addgene inc)
96
Addgene inc prk5 ha ubiquitin wt
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Prk5 Ha Ubiquitin Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/pRK5-HA-Ubiquitin-WT+(Plasmid+%2317608)/pmc12957317-270-16-17
Average 96 stars, based on 1 article reviews
prk5 ha ubiquitin wt - by Bioz Stars, 2026-10
96/100 stars
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cas9 2a gfp  (Addgene inc)
94
Addgene inc cas9 2a gfp
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Cas9 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/pCas9_GFP+(Plasmid+%2344719)/pmc09433428-30-12-19
Average 94 stars, based on 1 article reviews
cas9 2a gfp - by Bioz Stars, 2026-10
94/100 stars
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tnt quick coupled transcription/translation system  (Promega)
90
Promega tnt quick coupled transcription/translation system
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Tnt Quick Coupled Transcription/Translation System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/rabbit+reticulocyte+lysate/pm39562637-81-1-6
Average 90 stars, based on 1 article reviews
tnt quick coupled transcription/translation system - by Bioz Stars, 2026-10
90/100 stars
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ha peptide  (MBL Life science)
90
MBL Life science ha peptide
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Ha Peptide, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/ha+peptide/pmc08553338-37-4-7
Average 90 stars, based on 1 article reviews
ha peptide - by Bioz Stars, 2026-10
90/100 stars
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recombinant human il-1 beta/il-1f2 protein  (Bio-Techne corporation)
97
Bio-Techne corporation recombinant human il-1 beta/il-1f2 protein
PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with <t>HA-ubiquitin</t> and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.
Recombinant Human Il 1 Beta/Il 1f2 Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha-tagged+protein+cat%2E/Recombinant+Human+IL-1+beta%2FIL-1F2+Protein/custom%40201-lb%4034636324
Average 97 stars, based on 1 article reviews
recombinant human il-1 beta/il-1f2 protein - by Bioz Stars, 2026-10
97/100 stars
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Image Search Results


Journal: eLife

Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay

doi: 10.7554/eLife.71966

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , HA peptide , MBL Life science , Cat # 3320 , HA tagged Protein PURIFICATION KIT.

Techniques: Generated, CRISPR, Sequencing, Recombinant, Protein Purification, Western Blot, SYBR Green Assay, Enzyme-linked Immunosorbent Assay

PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.

Journal: International Journal of Molecular Sciences

Article Title: E3 Ubiquitin Ligase APC/C Cdh1 Regulation of Phenylalanine Hydroxylase Stability and Function

doi: 10.3390/ijms21239076

Figure Lengend Snippet: PAH is degraded by the 26S proteasomal pathway. ( A ) HEK293 cells were transfected transiently with HA-PAH and treated with increasing concentrations of MG132 and analyzed by Western blotting with the indicated antibodies. Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. The data shown here are the mean ± SD of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( B ) HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc or HA antibodies and immunoblotted with indicated antibodies. ( C ) Effect of MG132 on the ubiquitination and proteasomal degradation of Myc-PAH was determined. HEK293 cells were transfected with Myc-PAH along with HA-ubiquitin and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies. ( D ) K48- and K63-linked polyubiquitination of PAH. HEK293 cells were transfected with Myc-PAH along with HA-Ub-K48 and HA-Ub-K63 and immunoprecipitated with Myc antibody and immunoblotted with indicated antibodies.

Article Snippet: HA-tagged ubiquitin (Cat no. #18712), HA-tagged-ubiquitin-K48 (Cat no. #17605), and HA-tagged-ubiquitin-K63 (Cat no. #17606) were purchased from Addgene, Watertown, Massachusetts, USA.

Techniques: Transfection, Western Blot, Software, Immunoprecipitation

APC/C Cdh1 is the E3 ligase responsible for PAH protein degradation. ( A ) HEK293 cells were transfected with different E3 ligases along with ectopically expressing HA-PAH. Downregulation of PAH was analyzed by Western blotting. GAPDH was kept as a loading control. ( B ) HEK293 cells were transfected with a constant amount of HA-PAH (1 µg) along with increasing concentrations of Flag-Cdh1 (0, 0.25, 0.5, 1 µg). Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. Data were presented as the mean and standard deviation of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( C ) Effect of overexpression of Cdh1 on the ubiquitination and proteasomal degradation of HA-PAH was determined. HEK293 cells were transfected with HA-PAH, Flag-ubiquitin, and Flag-Cdh1 and immunoprecipitated with HA antibody and immunoblotted with indicated antibodies.

Journal: International Journal of Molecular Sciences

Article Title: E3 Ubiquitin Ligase APC/C Cdh1 Regulation of Phenylalanine Hydroxylase Stability and Function

doi: 10.3390/ijms21239076

Figure Lengend Snippet: APC/C Cdh1 is the E3 ligase responsible for PAH protein degradation. ( A ) HEK293 cells were transfected with different E3 ligases along with ectopically expressing HA-PAH. Downregulation of PAH was analyzed by Western blotting. GAPDH was kept as a loading control. ( B ) HEK293 cells were transfected with a constant amount of HA-PAH (1 µg) along with increasing concentrations of Flag-Cdh1 (0, 0.25, 0.5, 1 µg). Band intensity was estimated using ImageJ software, normalized with GAPDH, and represented graphically. Data were presented as the mean and standard deviation of three independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used, and p -values are indicated. ( C ) Effect of overexpression of Cdh1 on the ubiquitination and proteasomal degradation of HA-PAH was determined. HEK293 cells were transfected with HA-PAH, Flag-ubiquitin, and Flag-Cdh1 and immunoprecipitated with HA antibody and immunoblotted with indicated antibodies.

Article Snippet: HA-tagged ubiquitin (Cat no. #18712), HA-tagged-ubiquitin-K48 (Cat no. #17605), and HA-tagged-ubiquitin-K63 (Cat no. #17606) were purchased from Addgene, Watertown, Massachusetts, USA.

Techniques: Transfection, Expressing, Western Blot, Software, Standard Deviation, Over Expression, Immunoprecipitation

APC/C Cdh1 promotes phenylalanine metabolism. ( A ) HEK293 cells were transfected with HA-PAH alone or with Flag-ubiquitin, Flag-Cdh1, or sgRNA1 targeting Cdh1 . The ubiquitination of PAH was confirmed by co-immunoprecipitation with anti-HA antibody and immunoblotted with the antibodies indicated. ( B ) The transfection efficiency of respective constructs was confirmed by Western blot analysis. ( C ) Effect of Cdh1 depletion on phenylalanine metabolism using a phenylalanine assay kit. ( D ) Effect of Cdh1 depletion on phenylalanine metabolism using a Tyrosine assay kit. Data are presented as the mean and standard deviation of three independent experiments. One-way ANOVA followed by Dunnett’s test was used and p -values are indicated.

Journal: International Journal of Molecular Sciences

Article Title: E3 Ubiquitin Ligase APC/C Cdh1 Regulation of Phenylalanine Hydroxylase Stability and Function

doi: 10.3390/ijms21239076

Figure Lengend Snippet: APC/C Cdh1 promotes phenylalanine metabolism. ( A ) HEK293 cells were transfected with HA-PAH alone or with Flag-ubiquitin, Flag-Cdh1, or sgRNA1 targeting Cdh1 . The ubiquitination of PAH was confirmed by co-immunoprecipitation with anti-HA antibody and immunoblotted with the antibodies indicated. ( B ) The transfection efficiency of respective constructs was confirmed by Western blot analysis. ( C ) Effect of Cdh1 depletion on phenylalanine metabolism using a phenylalanine assay kit. ( D ) Effect of Cdh1 depletion on phenylalanine metabolism using a Tyrosine assay kit. Data are presented as the mean and standard deviation of three independent experiments. One-way ANOVA followed by Dunnett’s test was used and p -values are indicated.

Article Snippet: HA-tagged ubiquitin (Cat no. #18712), HA-tagged-ubiquitin-K48 (Cat no. #17605), and HA-tagged-ubiquitin-K63 (Cat no. #17606) were purchased from Addgene, Watertown, Massachusetts, USA.

Techniques: Transfection, Immunoprecipitation, Construct, Western Blot, Standard Deviation